A trans teenager, played by American trans actor Rhys Fehrenbacher, endures harassment from his classmates and comes face-to-face with his body dysphoria in gruesome visions.

Micah Lee, a trans man played by Chinese-American trans male actor Leo Sheng, explores relationships with men and women while also navigating infidelity, conflict in friendships, and biphobia.

Freddy McConnell, an English trans man, is filmed throughout the process of pausing testosterone hormone therapy in order to undergo artifical insemination and become pregnant, ultimately giving birth to a son. The documentary also addresses the hate and bigotry he experiences as a result.

Multiple trans people are filmed as they have gender-related surgery in America. This includes a non-binary person named Jordan, who has phalloplasty using a graft from their arm, and Shawn, a self-identified "stealth male" who undergoes an experimental type of phalloplasty intended to provide increased sensation and a different appearance.

Jake Rodriguez, a trans man played by American non-binary actor Garcia, tries to maintain a relationship with a lesbian woman while realising his increasing attraction to men. American trans actor Marquise Vilsón Balenciaga has a minor role.

Two English filmmakers interview trans and gender-diverse people in Russia, who discuss their experiences of transitioning, bigotry, pride, and being outed.

American trans man Tuck Mayo goes on a bachelor party road trip before his wedding, with his best friend Twiz Rimer accompanying him. Twiz is an American gender-variant person with Tourette’s syndrome.

The bisexual protagonist of this film falls in love with Katie, a gender non-conforming barista played by American non-binary actor Ro White.

Taylor Mason, a non-binary person played by American non-binary actor Asia Kate Dillon, is a highly intelligent businessperson who has sexual relationships with both men and women.

Rae Tutera, an American non-binary tailor, is filmed alongside their business partner as they make clothes for trans people and other gender-diverse individuals. Multiple trans men and non-binary people are interviewed.

Naveen Bhat, a non-binary person, is interviewed alongside their partner, their partner's family, and Indian LGBT+ activists, with the documentary focusing on the illegal detainment of Naveen in India by their parents, and their subsequent escape to the U.S.A.

Several American interviewees discuss their female masculinity, using the terms butch, gender-neutral, masculine-of-center, and genderqueer to describe themselves.

Butches, photographed and interviewed by American genderqueer butch SD Holman, provide insights into gender, sexuality, love, and butch diversity.

A child named Robin, played by Canadian actress Matreya Fedor, chooses to be known as a boy while attending swimming lessons. Inspired by a short story of the same name, published by butch/non-binary activist Ivan E. Coyote.

Multiple drag kings are interviewed about their motivations for doing drag, their identities, their medical transitions, and their understandings of gender. Interviewees include cis women, trans men, non-binary people, and an intersex non-binary person who is transitioning on testosterone and doesn't mind being read as a man.

Max Wolf Valerio, Texas Tomboy, and other trans people are interviewed about their gender identities, sexualities, and lives. Hida, an intersex person who was presumed female at birth, describes their non-binary experience and masculine experimentation.

Jamison Green, Loren Andrew Patrowski, Maxwell Scott Anderson, and several other American trans men are interviewed in this documentary. Eva Love, a trans woman, discusses her relationship with a trans man. Butch/trans trailblazer Leslie Feinberg is also interviewed. Dr. Toby Meltzer discusses phalloplasty and other transition surgeries.

Gaish, Kazuki, and Tatsu are three Onabe, which is a term used by some Japanese trans men and other Japanese gender-diverse people who were assigned female at birth. Tatsu, a trans man on testosterone, is interviewed alongside his cis female partner named Tomoe, and Kazuki is interviewed alongside his trans female partner named Kumi. Gaish describes themself as being neither a woman nor a man.